Informatics Publishing Limited

Kailash Nath Kaushik ¹, Saurabh Kumar Banerjee ¹

Affiliations
1 School of Pharmacy Technology and Management, SVKM’s, NMIMS University, Shirpur Campus, Shirpur-425405, Dist Dhule (M.S), IN

Abstract

A simple, accurate, precise and reproducible method for the estimation of domperidone (DOM) in tablet dosage form was developed utilizing concept of UV spectrophotometry. Methanol was used as the solvent for domperidone. The UV spectrum of domperidone in methanol showed λmax at 287.5 nm and Beer-Lambert law was obeyed in the concentration range of 6-36 μg/ml. Wavelength selected for estimation of domperidone was 287.5 nm. The result of analysis has been validated statistically. The recovery studies range from 99.5 to 100.1% confirmed the accuracy of the proposed methods.

Keywords

Domperidone, UV Spectrophotometry.

Full Text

     

Jagjitsingh C. Girase ¹, G. Venkateshwarlu ², Saurabh Kumar Banerjee ¹

Affiliations
1 School of Pharmacy Technology and Management, SVKM’s, NMIMS University, Shirpur Campus, Babulde, Near Bank of Tapi River, Shirpur-425405, Dist Dhule (M.S), IN
2 Research and Development Division, Concept Pharmaceuticals Ltd, Aurangabad, IN

Abstract

A reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Ceftriaxone Sodium and Sulbactam Sodium in bulk and Pharmaceutical Formulation. The separation was done by a Kromasil C18 column (250 mm×4.6 mm, 5 μm) using Acetonitrile: Buffer PH 7: Distilled Water (400:50:550, v/v/v) as mobile phase. The validation of the method was performed, and specificity, reproducibility, precision and accuracy were confirmed. The limit of detection was approximately 0.39 μg/ml for Ceftriaxone Sodium and 0.08 μg/ml for Sulbactam sodium. Due to simplicity and accuracy the method particularly suitable for routine pharmaceutical quality control.

Keywords

RP-HPLC, Ceftriaxone Sodium, Sulbactam Sodium.

Full Text